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FRAME Coordinate System and Calibration

Stage Coordinate System​

Origin Position​

The zero position of the XYZ stage corresponds to the A1 position of a standard multiwell plate. There is an offset between the virtual position displayed in ImSwitch and the actual physical position on the stage.

Calibration Target​

A calibration sample is provided to help locate the central reference point at coordinates xy = 63.5/46 mm:

Calibration target with crosshair lines to help navigate to the center position

The calibration target features lines that help users navigate to the center position and can also be used with fluorescence illumination.

Video demonstration link

Stage Travel Range​

The maximum travel range is 127 x 86 mm, suitable for multiwell plates:

Stage coordinate system showing maximum travel range

Axis Directions​

The coordinate system directions and orientations are defined as follows:

Detailed coordinate system showing axis directions and orientations

Calibration Procedures​

Stage Position Calibration​

  1. Load Calibration Target: Place the provided calibration sample on the stage
  2. Navigate to Center: Use the crosshair lines to navigate to position xy = 63.5/46 mm
  3. Record Offset: Note the difference between displayed and actual positions
  4. Update Configuration: Enter the offset values in the ImSwitch configuration

Optical Alignment​

For detailed optical alignment procedures, see:

Technical Specifications​

  • Coordinate Origin: Multiwell plate position A1
  • Calibration Point: 63.5/46 mm
  • Maximum Travel: 127 x 86 mm
  • Resolution: 0.3125 μm per step (configurable)
  • Repeatability: ±2 μm (typical)