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Introduction and Overview

What is the FRAME Microscope?​

The FRAME (Fast Rigid Automated Microscope Engine) represents a new approach to microscopy: an open, modular, automated, and flexibly adaptable microscope system. Based on the modular optics construction kit UC2 (You.See.Too.), the FRAME system lowers barriers to access complex optical and microscopy technology.

System Philosophy​

Modular Design Principles​

The FRAME system is built on complete modularization of all components regarding mechanics, electronics, and software:

  • Interchangeable Modules: Cube modules can be freely exchanged to switch between brightfield, fluorescence, or interference microscopy
  • Flexible Mechanics: Allows both classical objective setups and free arrangement for special geometries
  • Smart Electronics: Each cube can optionally have its own control unit for motors, galvo mirrors, or LEDs. All components share the same CAN BUS via 12V supply voltage.
  • Open Interface: System control via user-friendly graphical interface or programming interface (Python, REST, CAN BUS, USB)

Key Innovation Areas​

  1. Accessibility: Open-source hardware and software increase transparency, longevity, and interoperability
  2. Automation: Open system allows straightforward automation with built-in APIs for software control, camera integration, and laboratory automation
  3. Modularity: Instead of purchasing a completely new microscope, users can exchange or expand individual modules
  4. Cost Efficiency: Compared to proprietary complete systems, costs are reduced by 50-90% depending on configuration

System Overview​

Mechanical Foundation​

  • Robust Aluminum Frame: Provides stable foundation for precise measurements
  • Precision XYZ System: High-accuracy positioning and focusing mechanisms
  • Removable Optics Unit: Modular design allows easy reconfiguration
  • Slide-In System: Standardized 50mm cubes with quick-exchange capability
  • Linear Objective Revolver: Decide between two different magnifications/resolutions

Electronic Architecture​

  • CAN-BUS System: Smart networking of all functional units
  • Modular Controllers: ESP32 and Raspberry Pi-based control systems
  • Remote Operation: Web-based interface with cloud connectivity
  • IoT Integration: Network connectivity for remote monitoring and control

Software Ecosystem​

The FRAME system integrates with several software platforms:

Applications and Use Cases​

Research Applications​

  1. Digital Pathology: Automated image acquisition with AI analysis for early cancer detection
  2. Cell Biology/Live Cell Imaging: Long-term observation of living cell cultures in incubators
  3. Laboratory Automation: Integration with pipetting robots in diagnostics and drug screening
  4. Advanced Microscopy: Super-resolution techniques, FLIM, and specialized imaging modes

Industrial Applications​

  1. Quality Control: Materials inspection and defect analysis
  2. Semiconductor Industry: Wafer inspection and process control
  3. Biotechnology: Process monitoring and product validation
  4. Education: Modern imaging technique visualization in teaching and training

Advantages for Different User Groups​

Research Institutions​

  • Flexibility: Adapt system to specific research needs
  • Cost Efficiency: Lower initial investment and operational costs
  • Scalability: Add capabilities as research evolves
  • Community Support: Active user community and continuous development

Biotech Startups​

  • Low Entry Barrier: Affordable access to high-performance microscopy
  • Rapid Prototyping: Quick iteration on experimental setups
  • Modular Investment: Build system incrementally as funding allows
  • Open Standards: Avoid vendor lock-in

Educational Institutions​

  • Teaching Tool: Demonstrate modern imaging techniques
  • Student Projects: Hands-on experience with modular systems
  • Budget Friendly: Accessible pricing for educational budgets
  • Future Skills: Prepare students for open-source technologies

Sustainability and Environmental Impact​

Resource Conservation​

The modular approach contributes to sustainability on multiple levels:

  1. Repair & Maintenance: Instead of disposing of complete systems, only individual modules are replaced or adapted
  2. Upgrading: When components become outdated or new functions are needed, individual modules can be upgraded rather than replacing the entire system
  3. Local Production: Many mechanical components are produced locally via 3D printing using renewable materials

Open Source Benefits​

  • Global Accessibility: Electronic platforms can be replicated worldwide, including in resource-limited regions
  • Knowledge Sharing: Open documentation promotes global access to high technology
  • Community Development: Active community ensures continuous improvement and support
  • Local Repairs: Using 3D printing and digital fabrication, broken parts can be repaired on site

System Configurations​

Base Configuration​

The basic FRAME system includes:

  • Aluminum frame with XYZ positioning system
  • Basic cube modules for brightfield microscopy
  • Camera interface and basic illumination
  • Control electronics and software

Advanced Configurations​

Extended systems can include:

  • Multiple illumination modes (fluorescence, polarization, DIC)
  • Automated sample handling
  • Multi-camera setups
  • Environmental control (temperature, CO₂)
  • Integration with laboratory automation systems

Getting Started​

Before proceeding with installation and operation, ensure you have:

  1. Read all safety information in Section 1
  2. Verified system requirements detailed in Section 3
  3. Prepared installation space according to environmental specifications
  4. Organized necessary tools and accessories for assembly

The following sections will guide you through:

  • Technical specifications and requirements
  • Step-by-step installation procedures
  • Comprehensive operation instructions
  • Maintenance and troubleshooting guidance

Results from the Fluorescence Channels​

Continue to Technical Specifications for detailed system requirements and specifications.