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Technical Specifications

System Specifications​

System Overview​

Overall System Dimensions​

ParameterMotorized Version
Width250 mm
Depth250 mm
Height250 mm
Weight20 kg

Mechanical Specifications​

Frame Construction​

  • Material: Anodized aluminum profiles and plates
  • Vibration Damping: Integrated damping system (bumper feed)
  • Stage: Integrated XYZ Stage in one mechanical design
  • Objective Revolver: 2 Slots for multiple magnifications/resolutions (max diameter <28mm)

XYZ Positioning System​

  • X-Y Travel Range: up to 120 × 90 mm (Well plate format), stepsize: ~300nm
  • Z-Travel Range: 8 mm, stepsize: ~300nm
  • Resolution: 0.3 μm (motorized)
  • Accuracy: ±XXXX μm
  • Repeatability: ±0.5 μm

Optical Specifications​

Objectives Compatibility​

  • Thread: Standard RMS (20.32 mm × 36 TPI) and M25 × 0.75; customizable
  • Magnification Range: 2× to 100×
  • Numerical Aperture: Up to 1.4 (oil immersion)
  • Working Distance: 0.1 mm to 30 mm depending on objective
  • Correction: Infinity-corrected optics (160 mm and 200 mm tube length)
  • Customization: Bring your own microscope objective and design the adapter: XXXXX

Tube Lens System​

  • Focal Length: 100 mm (standard, customizable)
  • Resolution: Diffraction limited performance
  • Field Number: XXX

Condenser System​

  • Type: Koehler Illumination, LED Array using Neopixels
  • Numerical Aperture: 0.3 - 0.5 (LED array/Darkfield)
  • Aperture Control: Digitally using LED Ring addressing

Illumination System​

Transmitted Light​

  • LED Illumination:
    • White LED: 5000K, CRI >90
    • Power: 1W maximum
    • Intensity Control: 0-100% continuous via PWM and current control
    • Lifetime: >5,000 hours

Incident Light​

  • LED Ring Light: Adjustable segments for directional lighting
  • Fluorescence: Modular Laser system with multiple wavelengths
    • UV: 405nm
    • Blue: 488nm
    • Green: 530nm
    • Red: 635nm

Camera and Detection System​

Camera Interface​

  • Mount Type: C-mount
  • Sensor Compatibility: CMOS
  • Available Cameras: HIK IMX 179, IMX275
  • Connection: USB 3.0
  • Trigger: Hardware and software trigger support
  • Resolution: 1920 × 1200 to 5472 × 3648 pixels
  • Pixel Size: 2.4 μm to 6.5 μm
  • Frame Rate: Up to 200 fps (depending on resolution)
  • Sensitivity: >60% quantum efficiency
  • Dynamic Range: >60 dB

Electronic System​

Control Electronics​

  • Main Controller: Raspberry Pi 4 or ESP32-based system
  • Communication: CAN-BUS, USB, Ethernet, WiFi
  • I/O Interfaces: GPIO, SPI, I2C, UART
  • Power Supply: 24V DC, 5A (switching power supply included)

Motor Controllers​

  • Stepper Motors: NEMA8 (Z), Nema12 (X,Y,Objective), 200 Steps per Revolution, 1mm per revolution
  • Microstepping: Up to 256 microsteps per step (TMC2009)
  • Position Feedback: Magnetic encoders (for x,y - optional)
  • Homing: Optical limit switches

Sensor Integration​

  • Temperature: via I2C
  • Humidity: via I2C
  • Focus Sensor: optional: Astigmatism Based via CAN Bus (coming soon)

Software Specifications​

The internal Raspberry Pi 5 runs ImSwitch-OS which is a raspberry pi OS Sd card image that runs ImSwitch in a docker container. An external computer is needed to connect to this instance via Wifi. More to that later in the Operaional Manual

Operating System Compatibility​

More information about this here: https://openuc2.github.io/ XXX

  • Windows: Windows 10/11 (64-bit)
  • macOS: macOS 10.15 or later
  • Linux: Ubuntu 20.04 LTS or later
  • Web Interface: Modern browsers (Chrome, Firefox, Safari, Edge)

Software Requirements​

Frontend-only:

  • RAM: 8 GB minimum, 16 GB recommended
  • Storage: 1 GB free space for software, additional space for images
  • Graphics: OpenGL 3.0 compatible graphics card
  • Network: Ethernet or WiFi for remote control

Environmental Requirements​

Operating Conditions​

  • Temperature: 5°C to 38°C (59°F to 95°F)
  • Humidity: 30% to 80% relative humidity, non-condensing
  • Atmospheric Pressure: 86 kPa to 106 kPa
  • Altitude: Up to 2000 m above sea level

Storage Conditions​

  • Temperature: -10°C to 60°C (14°F to 140°F)
  • Humidity: 10% to 95% relative humidity, non-condensing

Installation Requirements​

  • Vibration: Isolated surface recommended for best performance
  • Space: 1 m × 1.5 m minimum work area
  • Power: 230V AC, 50/60 Hz (or 115V AC with adapter)
  • Ventilation: Natural convection (no forced ventilation required)

Component Specifications​

Inner Cube System​

Cube Drawer Mechanism​

  • Number of Positions: 4 cube positions (configurable)
  • Drawer Travel: 150mm sliding range per position
  • Drawer Rails: Precision linear ball bearing guides
  • Load Capacity: 500g per cube position
  • Positioning Accuracy: ±0.05mm repeatability
  • Locking Mechanism: Spring-loaded detent system

Cube Modules (Standard 50mm)​

Mechanical Specifications​

  • Dimensions: 50 × 50 × 50 mm
  • Material: Anodized aluminum or 3D-printed PLA/PETG
  • Precision: ±0.1 mm dimensional accuracy
  • Interface: Dovetail connection system
  • Mounting: Threaded inserts M3 and M4 compatible
  • Optical Aperture: 25mm clear aperture (configurable)

Electronic Components​

  • Microcontrollers: ESP32, Arduino-compatible (see https://github.com/youseetoo/uc2-esp32/)
  • Communication: I2C, SPI, CAN-BUS
  • Power: 12V input via CAN Bus, operational voltage 3.3V, 5V
  • Indicators: LED status lights via Neopixel interface (on board breakout)

Optical Specifications​

  • Aperture Diameter: 25mm standard (custom sizes available)
  • Coating: Anti-reflection coating on optical surfaces
  • Alignment Tolerance: ±0.02mm lateral, ±0.01mm axial
  • Surface Quality: 60-40 scratch-dig specification
  • Clear Aperture: >90% of geometric aperture

Stage System​

Motorized Stage​

  • Travel Range: 75 × 50 mm (standard), 150 × 100 mm (extended)
  • Resolution: 0.1 μm
  • Speed: 0.1 μm/s to 10 mm/s
  • Acceleration: Programmable, up to 100 mm/s²
  • Load Capacity: 2 kg maximum

Focus System​

Motorized Focus​

  • Range: 25 mm
  • Resolution: 0.05 μm
  • Speed: 0.1 μm/s to 5 mm/s
  • Repeatability: ±0.1 μm
  • Autofocus: Laser-based or image-based

Compatibility and Standards​

Microscopy Standards​

  • ISO 8038: Optics and photonics - Microscopes
  • DIN 58887: Microscopes - Objectives

Communication Standards​

  • CAN ISO-TP: Controller Area Network protocol
  • USB 3.0: Universal Serial Bus specification
  • Ethernet: IEEE 802.3 standard
  • WiFi: IEEE 802.11ac/ax standards

Safety Standards​

  • IEC 61010-1: Safety requirements for electrical equipment
  • IEC 62471: Photobiological safety of lamps and lamp systems
  • EN 55011: Electromagnetic compatibility

Performance Specifications​

Continue to Installation and Setup for detailed installation procedures.