Appendix
List of Abbreviations
| Abbreviation | Full Form | Description |
|---|---|---|
| AC | Alternating Current | Standard electrical power supply |
| API | Application Programming Interface | Software interface for system control |
| CAN | Controller Area Network | Communication protocol for devices |
| CCD | Charge-Coupled Device | Type of image sensor |
| CE | Conformité Européenne | European conformity marking |
| CMOS | Complementary Metal-Oxide-Semiconductor | Type of image sensor |
| CRI | Color Rendering Index | Measure of light quality |
| DC | Direct Current | Type of electrical current |
| DIC | Differential Interference Contrast | Microscopy technique |
| DIN | Deutsches Institut für Normung | German standards organization |
| EMI | Electromagnetic Interference | Unwanted electromagnetic radiation |
| ESP32 | Espressif Systems microcontroller | Control system component |
| FRAME | Fast Rigid Automated Microscope Engine | System name |
| GigE | Gigabit Ethernet | High-speed network interface |
| GPIO | General Purpose Input/Output | Programmable interface pins |
| I2C | Inter-Integrated Circuit | Communication protocol |
| IEC | International Electrotechnical Commission | Standards organization |
| IoT | Internet of Things | Network of connected devices |
| IP | Internet Protocol | Network communication standard |
| ISO | International Organization for Standardization | Standards organization |
| LED | Light Emitting Diode | Light source technology |
| NA | Numerical Aperture | Measure of lens light-gathering ability |
| PC | Personal Computer | Desktop or laptop computer |
| REST | Representational State Transfer | Web service architecture |
| RH | Relative Humidity | Moisture content measure |
| RMS | Royal Microscopical Society | Microscope thread standard |
| RoHS | Restriction of Hazardous Substances | Environmental regulation |
| SPI | Serial Peripheral Interface | Communication protocol |
| TPI | Threads Per Inch | Thread density measure |
| UC2 | You.See.Too. | Modular optics system |
| UART | Universal Asynchronous Receiver-Transmitter | Serial communication |
| USB | Universal Serial Bus | Computer interface standard |
| UV | Ultraviolet | Light wavelength range |
| WEEE | Waste Electrical and Electronic Equipment | Environmental regulation |
| WiFi | Wireless Fidelity | Wireless network technology |
| XYZ | Three-dimensional coordinates | Spatial positioning system |
Technical Glossary
A
Aperture Diaphragm: Adjustable opening that controls the numerical aperture of the condenser and affects image contrast and resolution.
Autofocus: Automated system for achieving optimal focus, typically using laser triangulation or image analysis algorithms.
B
Brightfield Microscopy: Standard microscopy technique using transmitted white light for sample illumination.
Back Focal Plane: The plane at the back of an objective lens where the aperture diaphragm image is formed.
C
CAN-BUS: Digital communication protocol that allows multiple devices to communicate on a single network.
Condenser: Optical system that focuses light onto the specimen for optimal illumination.
Cube Module: Standardized 50mm optical/electronic component that fits into the modular FRAME system.
D
DIC (Differential Interference Contrast): Advanced microscopy technique that enhances contrast in transparent specimens.
Depth of Field: The range of distances within which objects appear acceptably sharp in an image.
E
Encoder: Device that provides position feedback for motorized systems.
Extinction Ratio: Measure of polarizer efficiency, expressed as the ratio of transmitted light in perpendicular orientations.
F
Field Diaphragm: Adjustable aperture that controls the diameter of the illuminated field.
Focus Drift: Gradual change in focus position over time, typically due to thermal expansion.
FRAME: Fast Rigid Automated Microscope Engine - the name of the modular microscope system.
G
GPIO: General Purpose Input/Output pins on microcontrollers for interfacing with external devices.
H
Home Position: Reference position for motorized axes, typically set during system initialization.
I
ImSwitch: Advanced microscopy control software compatible with the FRAME system.
Infinity Optics: Optical system design where light rays are parallel between objective and tube lens.
K
Köhler Illumination: Optimal illumination method that provides even lighting and maximizes resolution.
L
Light Manager: Intelligent illumination control system with automatic optimization features.
Live View: Real-time display of camera images during operation.
M
Modular Design: System architecture allowing components to be easily exchanged or reconfigured.
Motorized System: Version of FRAME with computer-controlled movement and positioning.
N
Numerical Aperture (NA): Measure of a lens's light-gathering ability and resolving power.
O
Objective Lens: Primary magnifying lens closest to the specimen.
Open Source: Software or hardware designs freely available for modification and distribution.
P
Phase Contrast: Microscopy technique that converts phase differences to amplitude differences for enhanced contrast.
Pixel Calibration: Process of determining the physical size represented by each camera pixel.
Q
Quantum Efficiency: Measure of sensor sensitivity, expressed as percentage of incident photons converted to electrons.
R
Resolution: Smallest distance between two points that can be distinguished as separate.
REST API: Web service interface allowing remote control of system functions.
S
Stage: Platform that holds and positions specimens for observation.
Stepper Motor: Motor that moves in precise angular increments for accurate positioning.
T
Tube Lens: Lens that forms the final image in an infinity-corrected optical system.
Travel Range: Maximum distance of movement for motorized axes.
U
UC2: You.See.Too. - modular optics construction kit underlying the FRAME system.
USB: Universal Serial Bus interface for computer communication.
V
Vibration Isolation: Methods to minimize mechanical vibrations that can affect image quality.
W
Web Interface: Browser-based control interface for remote system operation.
Working Distance: Distance between objective front lens and specimen when in focus.
X, Y, Z
XYZ Positioning: Three-dimensional coordinate system for specimen positioning and movement.
Contact Information
Technical Support
Primary Contact:
- Company: openUC2 GmbH
- Address: [Company Address], Jena, Germany
- Phone: +49 (0) XXXX XXXXXX
- Email: support@openuc2.com
- Website: https://openuc2.github.io
Support Hours:
- Monday - Friday: 9:00 AM - 5:00 PM CET
- Emergency support available 24/7 for safety-related issues
Sales and Information
Sales Contact:
- Email: sales@openuc2.com
- Phone: +49 (0) XXXX XXXXXX
General Information:
- Email: info@openuc2.com
- Website: https://openuc2.github.io
Training and Education
Training Programs:
- Email: training@openuc2.com
- Workshops: Regular workshops available
- Online Training: Video tutorials and documentation
Regional Distributors
Contact openUC2 for information about authorized distributors in your region.
Additional Resources
Online Documentation
Main Documentation Site: https://openuc2.github.io
- Complete documentation library
- Software downloads
- Hardware specifications
- Community forums
GitHub Repositories:
- Hardware designs: https://github.com/openUC2
- Software packages: https://github.com/openUC2
- Example projects and tutorials
Software Resources
ImSwitch:
- Download: [ImSwitch GitHub]
- Documentation: [ImSwitch Docs]
- Community support
UC2-REST API:
- API documentation
- Example code
- Integration guides
Python Libraries:
- UC2-Python package
- Example scripts
- Automation tutorials
Community and Support
User Community:
- Discussion forums
- User-contributed content
- Project sharing platform
- Regular community meetings
Educational Resources:
- Video tutorials
- Webinar recordings
- Application notes
- Best practices guides
Standards and Compliance
Relevant Standards:
- ISO 8038: Optics and photonics - Microscopes
- IEC 61010-1: Safety requirements for electrical equipment
- EN 55011: Electromagnetic compatibility
- RoHS Directive 2011/65/EU
- WEEE Directive 2012/19/EU
Certification Bodies:
- CE marking through notified body
- ISO certification for quality management
- Environmental compliance verification
Warranty and Legal Information
Limited Warranty
openUC2 GmbH warrants this product to be free from defects in materials and workmanship under normal use for a period of one (1) year from the date of purchase.
Warranty Coverage:
- Parts and labor for manufacturing defects
- Software updates during warranty period
- Technical support
- Calibration verification
Warranty Exclusions:
- Damage from misuse or abuse
- Normal wear and tear
- Modifications without authorization
- Use outside specified environmental conditions
- Damage from unauthorized service
Limitation of Liability
openUC2 GmbH's liability is limited to repair or replacement of defective products. In no event shall openUC2 GmbH be liable for incidental or consequential damages.
Intellectual Property
This product may be covered by patents. For current patent information, contact openUC2 GmbH.
Open Source Components: Many components use open source licenses. For complete license information, see the software documentation.
Export Control
This product may be subject to export control regulations. Check local regulations before shipping internationally.
Document Revision History
| Version | Date | Changes | Author |
|---|---|---|---|
| 1.0 | June 2024 | Initial release | openUC2 Technical Team |
| Complete operational manual | |||
| CE compliance documentation | |||
| Multi-file structure for docusaurus |
Feedback and Improvements
We welcome feedback on this documentation. Please send suggestions to: documentation@openuc2.com
Areas for Future Updates:
- Additional application examples
- Advanced automation tutorials
- Troubleshooting case studies
- Integration guides for specific software
Index
A
- Alignment procedures, 67, 89
- Automation scripts, 134-137
- Autofocus system, 118-119
B
- Brightfield microscopy, 125-126
- Backup procedures, 158
C
- Calibration procedures, 150-154
- Camera setup, 67, 89
- CE compliance, 6-7
- Cleaning procedures, 146-149
- CAN-BUS communication, 56, 136
D
- Daily maintenance, 145
- DIC microscopy, 127
E
- Error codes, 142-143
- Environmental requirements, 47
F
- Focus system, 115-118
- Fluorescence microscopy, 127-128
- FRAME overview, 11-16
I
- Installation procedures, 64-90
- ImSwitch software, 112-114
M
- Software operation, 110-115
- Maintenance schedule, 145-146
- Motorized operation, 115-124
O
- Optical specifications, 45-46
- Operation procedures, 110-143
S
- Safety information, 6-10
- Software installation, 85-87
- Stage operation, 115, 119-120
- System startup, 110
T
- Technical specifications, 44-59
- Troubleshooting guide, 154-161
- Training resources, 165
W
- Warranty information, 166
End of Document
© openUC2 GmbH. All rights reserved. This document contains proprietary information and is provided for the operation of the FRAME Microscope system only.
Document Information:
- Document Version: 1.0
- Publication Date: June 2024
- Language: English
- Total Pages: 7 sections
- Format: Multi-file structure for docusaurus integration
For the latest updates and additional resources, visit: https://openuc2.github.io