openUC2 FiveD v4

Design Files

:::warn Coming soon-ish :::

Version 4

This is a novel version of the XYZ microscope that also includes fluorescence imaging. It is not based on the UC2 cubes anymore but featues a monolithic body that is not made out of metal sheet!

More information are comming soon!

Introduction

A first video explains the basic operation of the device

Software

The device runs using ImSwitch with the following Configuration. Find the ImSwitch Installer here: https://github.com/openUC2/ImSwitchInstaller/releases/tag/v0.0.2 (go to latest release)

Config

{
  "positioners": {
    "ESP32Stage": {
      "managerName": "ESP32StageManager",
      "managerProperties": {
        "rs232device": "ESP32",
        "isEnable": true,
        "enableauto": false,
        "stepsizeX": -0.3125,
        "stepsizeY": -0.3125,
        "stepsizeZ": 0.3125,
        "homeSpeedX": 15000,
        "homeSpeedY": 15000,
        "homeSpeedZ": 15000,
        "isDualaxis": true,
        "homeDirectionX": 1,
        "backlashXOld": 15,
        "backlashYOld": 40,
        "backlashX": 0,
        "backlashY": 0,
        "homeEndstoppolarityY": 0,
        "homeDirectionY": -1,
        "homeDirectionZ": 0,
        "homeXenabled": 1,
        "homeYenabled": 1,
        "homeZenabled": 0,
        "initialSpeed": {
          "X": 15000,
          "Y": 15000,
          "Z": 15000
        }
      },
      "axes": [
        "X",
        "Y",
        "Z"
      ],
      "forScanning": true,
      "forPositioning": true
    }
  },
  "rs232devices": {
    "ESP32": {
      "managerName": "ESP32Manager",
      "managerProperties": {
        "host_": "192.168.43.129",
        "serialport": "COM5",
        "baudrate":115200,
        "debug":1
      }
    }
  },
  "lasers": {
    "LED": {
        "analogChannel": null,
        "digitalLine": null,
        "managerName": "ESP32LEDLaserManager",
        "managerProperties": {
          "rs232device": "ESP32",
          "channel_index": 1
        },
        "wavelength": 0,
        "valueRangeMin": 0,
        "valueRangeMax": 1023
      },
      "Laser 488": {
        "analogChannel": null,
        "digitalLine": null,
        "managerName": "ESP32LEDLaserManager",
        "managerProperties": {
          "rs232device": "ESP32",
          "channel_index": 2
        },
        "wavelength": 635,
        "valueRangeMin": 0,
        "valueRangeMax": 1023
      },
      "Laser 635": {
        "analogChannel": null,
        "digitalLine": null,
        "managerName": "ESP32LEDLaserManager",
        "managerProperties": {
          "rs232device": "ESP32",
          "channel_index": 3
        },
        "wavelength": 488,
        "valueRangeMin": 0,
        "valueRangeMax": 1023
      }

    },
  "detectors": {
    "WidefieldCamera": {
      "analogChannel": null,
      "digitalLine": null,
      "managerName": "HikCamManager",
      "managerProperties": {
        "isRGB": 1,
        "cameraListIndex": 0,
        "cameraEffPixelsize": 0.2257,
        "hikcam": {
          "exposure": 0,
          "gain": 0,
          "blacklevel": 100,
          "image_width": 1000,
          "image_height": 1000
        }
      },
      "forAcquisition": true,
      "forFocusLock": true
    },
    "Observer": {
      "analogChannel": null,
      "digitalLine": null,
      "managerName": "OpenCVCamManager",
      "managerProperties": {
        "cameraListIndex": 1,
        "cameraListIndexWIN": 0,
        "isRGB":1,
        "opencvcam": {
          "exposure": 10
        }
      },
      "forAcquisition": true
    }
  },
  "autofocus": {
    "camera": "WidefieldCamera",
    "positioner": "ESP32Stage",
    "updateFreq": 10,
    "frameCropx": 780,
    "frameCropy": 400,
    "frameCropw": 500,
    "frameCroph": 100
  },
  "mct": {
    "monitorIdx": 2,
    "width": 1080,
    "height": 1920,
    "wavelength": 0,
    "pixelSize": 0,
    "angleMount": 0,
    "patternsDirWin": "C:\\Users\\wanghaoran\\Documents\\ImSwitchConfig\\imcontrol_slm\\488\\",
    "patternsDir": "/users/bene/ImSwitchConfig/imcontrol_sim/488"
  },
  "PixelCalibration": {},
  "focusLock": {
    "camera": "WidefieldCamera",
    "positioner": "ESP32StageManager",
    "updateFreq": 4,
    "frameCropx": 0,
    "frameCropy": 0,
    "frameCropw": 0,
    "frameCroph": 0
  },
  "availableWidgets": [
    "Settings",
    "View",
    "Recording",
    "Image",
    "Laser",
    "Positioner",
    "Autofocus",
    "MCT",
    "UC2Config",
    "ImSwitchServer",
    "PixelCalibration",
    "HistoScan",
    "ROIScan"
  ],
  "nonAvailableWidgets": [
    "STORMRecon",
    "DPC",
    "Hypha",
    "FocusLock",
    "HistoScan",
    "FocusLock",
    "FOVLock"
  ]
}

Assembly Instructions

Assembly of the Optics Cube

Necessary Parts to assemble the Optics module: Two halfs to mount the optics/beamsplitters, two lids and screws

Add mirror to the beamsplitter component

Add the mirror + objective lens to the assembly (ensure the focus cannot move after you insert the lens; fixing the lens is not necessary as we want to rotate the field of view eventually)

Close the two halfs and screw them together with M3x25mm; (old version: add the 12mmx1mm rods for the sliding mechanism by glueing them)

Fully Assembled module with locking mechanism (metal rods)

Fluorescent Cube

Fluorescent Cube inside Optics Module

Inserting the Modules

This shows how the optics module have to be slided into the FiveD assembly. There is a series of groves, slides and accompanying spring-loaded mechanisms to keep the module in exactly one place. It is mechanically well defined. The way to slide it in is a bit involved, but worth it:

one of the positioning spheres (without spring) beaks first