Assemble the XYZ Microscope

Bill of Material

This is exported from Inventor

Assembly Procedure

Preparing the XY Stage

A first step is to prepare the XY stage such that it adapts the linear motors.

TODO:

have sketch for drilling holes

Tools

Find the necessary tools to get rid of the necessary parts of the stage:

Philips screwdriver at various sizes (small, coarse)
slotted screwdriver at various sizes (small, coarse)
Pliers "Kneifzange"

Steps

Please collect all parts in the bag that comes with the XY stage. First step: Remove ALL the screws on the up-facing surce (mostly cross)

Get rid of the sample clamps using the Philips 1 screwdriver (cross)

Remove the metal bars that frame the glass plate

Remove the glass plate - don't cut yourself!

Take the slotted screwdriver (small) and remove the cap of the knob (there is a hole, insert it and remove the plastic piece)

You end up with this result:

Take the same slotted screw driver and remove the stickers that indicate the ruler signs

The remaining residuals can be ignored.

Take the small Philips (PH-1) and remove all screws on the xy deck

Remove the metal plate from the deck:

Take the 7mm nut and the ratchet to remove the M4 nut:

Remove the cap/knob by untwisting it counterclockwise:

Unscrew the small screws (3x) that hold the lower knob using the Philips (PH1)

Remove the lower hand knob:

Turn over the stage

Remove the larger cross-headed screws with the larger Philips (PH2) from the mounting ring:

Remove the ring

Turn over the stage again: Use the slotted screwdriver (2mm) to get rid of the screws (2x) in the lower knob (setscrews)

Remove all screws of the linear rack (linear gear) using Philips (PH1)

Remove the metal part:

Same with the gear on the lower end

Remove the part:

Unscrew the special-shaped nut. Attention: this holds lubricon, make sure you cover your fingers. Use papertowels to cover the part / your fingers.

Eventually take pliers to release the nut

Remove the round metal plate by pulling it up (use paper towels!!)

Use the small Philips and remove the screws along the side and remove the metal part

Remove the screws inside the assembly in order to enlarge the travel range (PH1)

Take the wrench (12mm) and loosen the special-shaped screw assembly. Hold the lower nut and unscrew it counter-clockwise. Fully remove the screw using the papertowel by hand - all the way off.

It's not 100% straight forward, but it'll work out:

Tidy up the workplace:

Add drill holes to the XY Stage assembly

For this we will have a template that indicate all holes and their sizes soon! For now we will add the parts manually as shown below:

Tools

Bosch Ständerbohrmaschine WPD40
Drills: 2.8mm
Drills: 4.7mm
Tap: M3
Tap: 4
openUC2 drill template
pen

Parts

prepared xy stage
NEMA12 100mm linear stage
NEMA12 150mm linear stage

Steps

Take the 150mm stage and hold it on the plate as shown in the photo. Mark the holes that will mount the stage on the xy plate_

Same for the other (100mm) linear stage:

Add markers for the positioning on the xy stage

Quick check:

Take the Ständerbohrmaschine and use a drill for the M3 Tap. Make through holes everywhere, where you marked the spots. Use safety goggles:

Remove dust with a vacuum cleaner

Cut taps. Make sure holes are clearn and through! Mount the linear motorized stage on the xy stage with M3 cylindrical screws (DIN908, 8mm)

Mount stage on the other side as well:

We also need to add the M5 long-rods (155mm). They have to fit through the holes of the uc2 mounting plate (see last step)

The metal-parts are done

Adding the auxilary/3D-printed parts

The remaining parts will first connect the linear motors to the stage in order to move it around and second, add a plate that mounts the stage to the cubes.

Add the mounting arm on the upper part:

Add the mounting arm on the lower part:

Equip the Plate with the Illumination adapter (which side depends on the later assembly - if not fluorescence, it doesn't matter) The M5 nuts are not necessary anymore. The remaining holes help you guiding the M5 rodsinto the holes. (see former photo) Add M3 screws to the plate from above such that you have open-ended threads lurking from the other side (hardly visible TODO: add image!)

Fixate the illumination arm holder with the metal stage using an M3 hole (eventually drill a hole 2.7mm)

Screw the plate to the M3 screw holes on the xy stage (bottom part). The stage should look like this. Now, we can continue to the next step.

Prepare the Z-stage

The documentation for the motorized 25mm Z-stage can be found here: https://openuc2.github.io/docs/PRODUCTION/PG_12_STAGE_Z_NEMA

Once done, lock the Stage with the Puzzle piece with M5x8 worm screws:

Prepare Electronics

Bill of Material

UC2e v2 electronics
3x A4988 Stepper driver
12V power supply
USB micro cable
3D printed case
2x puzzle pieces
8 M5x8 thread-only screws
4x M3x8mm screws

Assembly

Attach the electronics board to the 3D printed assembly and tighten it with the M3 screws (cylindrical, Din906) Attach the puzzle pieces to the distal ends of the assembly and lock it with the M5 screws. For this the yet closed holes have to be opened by "drilling" it through.

Prepare Triangle Structure

Tubelens

Bill of Material

Berrybase 100mm CCTV Lens
Daheng Vision IMX226 sensor
USB 3 Camera Cable
2x Puzzlepieze
8x M5x8 mm worm screw
4x M3x18mm screw

Assembly

Adding the Baseplate

Endstops and Illumination

Skeleton

Fully Assembled

Fluo Extension

Improvements

Stage does not run smoothly

You can release the pressure on the linear bearings by loosening the screws carefully. Make sure you don't introduce unneccesary play. The stage works with two v-grooves and balls in between.