Skip to main content

Discovery Fluorescence Box

The Discovery Fluorescence Box enables you to explore the fascinating world of fluorescence microscopy. Using laser excitation, you can visualize fluorescent molecules and structures that are invisible under conventional illumination.

What is Fluorescence?​

Fluorescence is a photophysical process where electrons are excited to a higher energy state by absorbing light at one wavelength (excitation) and then emit light at a longer wavelength (emission) as they return to the ground state. The difference in wavelengths, known as the Stokes shift, allows selective detection of fluorescent signals.

What You'll Learn​

  • Principles of fluorescence and photophysics
  • Laser-based excitation systems
  • Fluorescence filter sets and beam splitters
  • Stokes shift and emission spectra
  • Smart microscope automation with motorized Z-stage
  • Bright-field and fluorescence imaging combination

Experiments in this Section​

  • LASER Fluorescence Smart Microscope - Build an automated laser-powered fluorescence microscope
  • Fluorescence Smart Microscope - LED-based fluorescence imaging setup
  • LED Microscope Alignment - Detailed alignment procedures

Key Capabilities​

  • Laser-induced fluorescence imaging
  • Automated Z-stage focusing
  • PS4 controller operation
  • Combined bright-field and fluorescence imaging
  • Infinity-corrected optics

Required Components​

  • Laser diode (typically 488nm or 532nm)
  • Beam splitter with fluorescence filter
  • LED matrix for bright-field microscopy
  • Electronic Z-stage with motorized control
  • Mainboard with USB connection
  • PS4 controller
  • Infinity objective lens
  • Camera with tube lens
  • UC2 cubes and baseplates

Perfect for biology students, researchers, and anyone interested in exploring fluorescent samples at the microscopic level!