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XYZ Stage Control

Learn how to build, control, and integrate motorized XYZ stages into your UC2 microscopy system. Motorized stages enable automated scanning, focus stacking, and precise sample positioning.

Overview​

XYZ stages provide three-axis motorized control for:

  • X-axis: Lateral movement (left/right)
  • Y-axis: Lateral movement (forward/backward)
  • Z-axis: Vertical movement (focusing)

These stages are essential for automated microscopy, allowing programmatic control of sample position and focus.

What You'll Learn​

  • XYZ stage assembly and wiring
  • Stepper motor control principles
  • Stage calibration and homing
  • Software control via UC2 firmware
  • Integration with PS4 controller
  • Automated scanning patterns

Tutorials in this Section​

  • XYZ Stage Tutorial - Complete guide to building and controlling 3-axis motorized stages

Key Components​

  • Stepper Motors: NEMA 11 or NEMA 17 for precise positioning
  • Linear Rails: Guide smooth movement
  • Lead Screws: Convert rotation to linear motion
  • Motor Drivers: A4988 or DRV8825 for motor control
  • UC2 Standalone Board: Central control unit
  • Limit Switches: Optional homing and safety stops

Applications​

Research​

  • Automated tile scanning for large samples
  • 3D volume acquisition (z-stacks)
  • Time-lapse with position tracking
  • Multi-position imaging experiments

Education​

  • Teaching automation principles
  • Demonstrating precision mechanics
  • Exploring 3D microscopy
  • Learning stepper motor control

Control Methods​

Manual Control​

  • PS4 controller for real-time positioning
  • Web interface via WiFi
  • Serial commands via USB

Automated Control​

  • Predefined scanning patterns
  • Python scripting via serial
  • ImSwitch software integration
  • Custom automation scripts

Technical Capabilities​

  • Resolution: Micrometer-level positioning
  • Speed: Adjustable from slow precision to fast scanning
  • Range: Typically 20-100mm per axis
  • Repeatability: High precision for repeated positioning
  • Backlash Compensation: Software-controllable

Integration Examples​

XYZ stages are used in:

  • Light sheet microscopy (sample scanning)
  • Automated fluorescence imaging
  • Focus stacking for extended depth of field
  • Tile scanning for large sample areas
  • Automated well-plate imaging

Perfect for anyone building automated microscopy systems with precise sample positioning!